Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice. / Fromont-Racine, M; Bucchini, D; Madsen, O; Desbois, P; Linde, S; Saulnier, C; Ripoche, M A; Jami, J; Pictet, R; Nielsen, Jens Høiriis.

I: Molecular endocrinology (Baltimore, Md.), Bind 4, Nr. 5, 05.1990, s. 669-77.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Fromont-Racine, M, Bucchini, D, Madsen, O, Desbois, P, Linde, S, Saulnier, C, Ripoche, MA, Jami, J, Pictet, R & Nielsen, JH 1990, 'Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice', Molecular endocrinology (Baltimore, Md.), bind 4, nr. 5, s. 669-77.

APA

Fromont-Racine, M., Bucchini, D., Madsen, O., Desbois, P., Linde, S., Saulnier, C., ... Nielsen, J. H. (1990). Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice. Molecular endocrinology (Baltimore, Md.), 4(5), 669-77.

Vancouver

Fromont-Racine M, Bucchini D, Madsen O, Desbois P, Linde S, Saulnier C o.a. Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice. Molecular endocrinology (Baltimore, Md.). 1990 maj;4(5):669-77.

Author

Fromont-Racine, M ; Bucchini, D ; Madsen, O ; Desbois, P ; Linde, S ; Saulnier, C ; Ripoche, M A ; Jami, J ; Pictet, R ; Nielsen, Jens Høiriis. / Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice. I: Molecular endocrinology (Baltimore, Md.). 1990 ; Bind 4, Nr. 5. s. 669-77.

Bibtex

@article{ec58776ac9df4d13be5c7b3126dd6004,
title = "Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice",
abstract = "Expression of the human insulin gene was examined in transgenic mouse lines carrying the gene with various lengths of DNA sequences 5' to the transcription start site (+1). Expression of the transgene was demonstrated by 1) the presence of human C-peptide in urine, 2) the presence of specific transcripts in pancreas, but not in other tissues, 3) the specific immunofluorescence staining of pancreatic islets for human C-peptide, and 4) the synthesis and accumulation of human (pro)insulin in isolated islets. Deletions in the injected DNA fragment of sequences upstream from positions -353, -258, and -168 allowed correct initiation of the transcripts and cell specificity of expression, while quantitative expression gradually decreased. Deletion to -58 completely abolished the expression of the gene. The amount of human product that in mice harboring the longest fragment contributes up to 50{\%} of the total insulin does not alter the normal proportion of mice insulins I and II. These results suggest that expression of the human insulin gene in vivo results from the cooperation of several cis-regulatory elements present in the various deleted fragments. With none of the deletions used, expression of the transgene was observed in cell types other than beta-islet cells.",
keywords = "Animals, C-Peptide, Chromosome Deletion, Female, Gene Expression, Humans, Immunohistochemistry, Insulin, Male, Mice, Mice, Transgenic, Pancreas, RNA, Messenger",
author = "M Fromont-Racine and D Bucchini and O Madsen and P Desbois and S Linde and C Saulnier and Ripoche, {M A} and J Jami and R Pictet and Nielsen, {Jens H{\o}iriis}",
year = "1990",
month = "5",
language = "English",
volume = "4",
pages = "669--77",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice

AU - Fromont-Racine, M

AU - Bucchini, D

AU - Madsen, O

AU - Desbois, P

AU - Linde, S

AU - Saulnier, C

AU - Ripoche, M A

AU - Jami, J

AU - Pictet, R

AU - Nielsen, Jens Høiriis

PY - 1990/5

Y1 - 1990/5

N2 - Expression of the human insulin gene was examined in transgenic mouse lines carrying the gene with various lengths of DNA sequences 5' to the transcription start site (+1). Expression of the transgene was demonstrated by 1) the presence of human C-peptide in urine, 2) the presence of specific transcripts in pancreas, but not in other tissues, 3) the specific immunofluorescence staining of pancreatic islets for human C-peptide, and 4) the synthesis and accumulation of human (pro)insulin in isolated islets. Deletions in the injected DNA fragment of sequences upstream from positions -353, -258, and -168 allowed correct initiation of the transcripts and cell specificity of expression, while quantitative expression gradually decreased. Deletion to -58 completely abolished the expression of the gene. The amount of human product that in mice harboring the longest fragment contributes up to 50% of the total insulin does not alter the normal proportion of mice insulins I and II. These results suggest that expression of the human insulin gene in vivo results from the cooperation of several cis-regulatory elements present in the various deleted fragments. With none of the deletions used, expression of the transgene was observed in cell types other than beta-islet cells.

AB - Expression of the human insulin gene was examined in transgenic mouse lines carrying the gene with various lengths of DNA sequences 5' to the transcription start site (+1). Expression of the transgene was demonstrated by 1) the presence of human C-peptide in urine, 2) the presence of specific transcripts in pancreas, but not in other tissues, 3) the specific immunofluorescence staining of pancreatic islets for human C-peptide, and 4) the synthesis and accumulation of human (pro)insulin in isolated islets. Deletions in the injected DNA fragment of sequences upstream from positions -353, -258, and -168 allowed correct initiation of the transcripts and cell specificity of expression, while quantitative expression gradually decreased. Deletion to -58 completely abolished the expression of the gene. The amount of human product that in mice harboring the longest fragment contributes up to 50% of the total insulin does not alter the normal proportion of mice insulins I and II. These results suggest that expression of the human insulin gene in vivo results from the cooperation of several cis-regulatory elements present in the various deleted fragments. With none of the deletions used, expression of the transgene was observed in cell types other than beta-islet cells.

KW - Animals

KW - C-Peptide

KW - Chromosome Deletion

KW - Female

KW - Gene Expression

KW - Humans

KW - Immunohistochemistry

KW - Insulin

KW - Male

KW - Mice

KW - Mice, Transgenic

KW - Pancreas

KW - RNA, Messenger

M3 - Journal article

VL - 4

SP - 669

EP - 677

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 5

ER -

ID: 47974095