Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes.

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Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. / Albrechtsen, Nicolai Jacob Wewer; Kuhre, Rune Ehrenreich; Windeløv, Johanne Agerlin; Ørgaard, Anne; Deacon, Carolyn F.; Kissow, Hannelouise; Hartmann, Bolette; Holst, Jens Juul.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 311, Nr. 2, 2016, s. E302-E309.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Albrechtsen, NJW, Kuhre, RE, Windeløv, JA, Ørgaard, A, Deacon, CF, Kissow, H, Hartmann, B & Holst, JJ 2016, 'Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes.', American Journal of Physiology: Endocrinology and Metabolism, bind 311, nr. 2, s. E302-E309. https://doi.org/10.1152/ajpendo.00119.2016

APA

Albrechtsen, N. J. W., Kuhre, R. E., Windeløv, J. A., Ørgaard, A., Deacon, C. F., Kissow, H., Hartmann, B., & Holst, J. J. (2016). Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. American Journal of Physiology: Endocrinology and Metabolism, 311(2), E302-E309. https://doi.org/10.1152/ajpendo.00119.2016

Vancouver

Albrechtsen NJW, Kuhre RE, Windeløv JA, Ørgaard A, Deacon CF, Kissow H o.a. Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. American Journal of Physiology: Endocrinology and Metabolism. 2016;311(2):E302-E309. https://doi.org/10.1152/ajpendo.00119.2016

Author

Albrechtsen, Nicolai Jacob Wewer ; Kuhre, Rune Ehrenreich ; Windeløv, Johanne Agerlin ; Ørgaard, Anne ; Deacon, Carolyn F. ; Kissow, Hannelouise ; Hartmann, Bolette ; Holst, Jens Juul. / Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. I: American Journal of Physiology: Endocrinology and Metabolism. 2016 ; Bind 311, Nr. 2. s. E302-E309.

Bibtex

@article{d4fcd5b0b660406cbeacd12bf2e60fe6,
title = "Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes.",
abstract = "Glucagon is a metabolically important hormone, but many aspects of its physiology remain obscure, because glucagon secretion is difficult to measure in mice and rats due to methodological inadequacies. Here, we introduce and validate a low-volume, enzyme-linked immunosorbent glucagon assay according to current analytical guidelines, including tests of sensitivity, specificity, and accuracy, and compare it, using the Bland-Altman algorithm and size-exclusion chromatography, with three other widely cited assays. After demonstrating adequate performance of the assay, we measured glucagon secretion in response to intravenous glucose and arginine in anesthetized mice (isoflurane) and rats (Hypnorm/midazolam). Glucose caused a long-lasting suppression to very low values (1–2 pmol/l) within 2 min in both species. Arginine stimulated secretion 8- to 10-fold in both species, peaking at 1–2 min and returning to basal levels at 6 min (mice) and 12 min (rats). d-Mannitol (osmotic control) was without effect. Ketamine/xylazine anesthesia in mice strongly attenuated (P < 0.01) α-cell responses. Chromatography of pooled plasma samples confirmed the accuracy of the assay. In conclusion, dynamic analysis of glucagon secretion in rats and mice with the novel accurate sandwich enzyme-linked immunosorbent assay revealed extremely rapid and short-lived responses to arginine and rapid and profound suppression by glucose.",
author = "Albrechtsen, {Nicolai Jacob Wewer} and Kuhre, {Rune Ehrenreich} and Windel{\o}v, {Johanne Agerlin} and Anne {\O}rgaard and Deacon, {Carolyn F.} and Hannelouise Kissow and Bolette Hartmann and Holst, {Jens Juul}",
year = "2016",
doi = "10.1152/ajpendo.00119.2016",
language = "English",
volume = "311",
pages = "E302--E309",
journal = "American Journal of Physiology: Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "2",

}

RIS

TY - JOUR

T1 - Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes.

AU - Albrechtsen, Nicolai Jacob Wewer

AU - Kuhre, Rune Ehrenreich

AU - Windeløv, Johanne Agerlin

AU - Ørgaard, Anne

AU - Deacon, Carolyn F.

AU - Kissow, Hannelouise

AU - Hartmann, Bolette

AU - Holst, Jens Juul

PY - 2016

Y1 - 2016

N2 - Glucagon is a metabolically important hormone, but many aspects of its physiology remain obscure, because glucagon secretion is difficult to measure in mice and rats due to methodological inadequacies. Here, we introduce and validate a low-volume, enzyme-linked immunosorbent glucagon assay according to current analytical guidelines, including tests of sensitivity, specificity, and accuracy, and compare it, using the Bland-Altman algorithm and size-exclusion chromatography, with three other widely cited assays. After demonstrating adequate performance of the assay, we measured glucagon secretion in response to intravenous glucose and arginine in anesthetized mice (isoflurane) and rats (Hypnorm/midazolam). Glucose caused a long-lasting suppression to very low values (1–2 pmol/l) within 2 min in both species. Arginine stimulated secretion 8- to 10-fold in both species, peaking at 1–2 min and returning to basal levels at 6 min (mice) and 12 min (rats). d-Mannitol (osmotic control) was without effect. Ketamine/xylazine anesthesia in mice strongly attenuated (P < 0.01) α-cell responses. Chromatography of pooled plasma samples confirmed the accuracy of the assay. In conclusion, dynamic analysis of glucagon secretion in rats and mice with the novel accurate sandwich enzyme-linked immunosorbent assay revealed extremely rapid and short-lived responses to arginine and rapid and profound suppression by glucose.

AB - Glucagon is a metabolically important hormone, but many aspects of its physiology remain obscure, because glucagon secretion is difficult to measure in mice and rats due to methodological inadequacies. Here, we introduce and validate a low-volume, enzyme-linked immunosorbent glucagon assay according to current analytical guidelines, including tests of sensitivity, specificity, and accuracy, and compare it, using the Bland-Altman algorithm and size-exclusion chromatography, with three other widely cited assays. After demonstrating adequate performance of the assay, we measured glucagon secretion in response to intravenous glucose and arginine in anesthetized mice (isoflurane) and rats (Hypnorm/midazolam). Glucose caused a long-lasting suppression to very low values (1–2 pmol/l) within 2 min in both species. Arginine stimulated secretion 8- to 10-fold in both species, peaking at 1–2 min and returning to basal levels at 6 min (mice) and 12 min (rats). d-Mannitol (osmotic control) was without effect. Ketamine/xylazine anesthesia in mice strongly attenuated (P < 0.01) α-cell responses. Chromatography of pooled plasma samples confirmed the accuracy of the assay. In conclusion, dynamic analysis of glucagon secretion in rats and mice with the novel accurate sandwich enzyme-linked immunosorbent assay revealed extremely rapid and short-lived responses to arginine and rapid and profound suppression by glucose.

U2 - 10.1152/ajpendo.00119.2016

DO - 10.1152/ajpendo.00119.2016

M3 - Journal article

C2 - 27245336

VL - 311

SP - E302-E309

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

IS - 2

ER -

ID: 162894373