Diversity of conotoxin gene superfamilies in the venomous snail, Conus victoriae
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Diversity of conotoxin gene superfamilies in the venomous snail, Conus victoriae. / Robinson, Samuel D; Safavi-Hemami, Helena; McIntosh, Lachlan D; Purcell, Anthony W; Norton, Raymond S; Papenfuss, Anthony T.
I: PLoS ONE, Bind 9, Nr. 2, e87648, 2014.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Diversity of conotoxin gene superfamilies in the venomous snail, Conus victoriae
AU - Robinson, Samuel D
AU - Safavi-Hemami, Helena
AU - McIntosh, Lachlan D
AU - Purcell, Anthony W
AU - Norton, Raymond S
AU - Papenfuss, Anthony T
PY - 2014
Y1 - 2014
N2 - Animal venoms represent a vast library of bioactive peptides and proteins with proven potential, not only as research tools but also as drug leads and therapeutics. This is illustrated clearly by marine cone snails (genus Conus), whose venoms consist of mixtures of hundreds of peptides (conotoxins) with a diverse array of molecular targets, including voltage- and ligand-gated ion channels, G-protein coupled receptors and neurotransmitter transporters. Several conotoxins have found applications as research tools, with some being used or developed as therapeutics. The primary objective of this study was the large-scale discovery of conotoxin sequences from the venom gland of an Australian cone snail species, Conus victoriae. Using cDNA library normalization, high-throughput 454 sequencing, de novo transcriptome assembly and annotation with BLASTX and profile hidden Markov models, we discovered over 100 unique conotoxin sequences from 20 gene superfamilies, the highest diversity of conotoxins so far reported in a single study. Many of the sequences identified are new members of known conotoxin superfamilies, some help to redefine these superfamilies and others represent altogether new classes of conotoxins. In addition, we have demonstrated an efficient combination of methods to mine an animal venom gland and generate a library of sequences encoding bioactive peptides.
AB - Animal venoms represent a vast library of bioactive peptides and proteins with proven potential, not only as research tools but also as drug leads and therapeutics. This is illustrated clearly by marine cone snails (genus Conus), whose venoms consist of mixtures of hundreds of peptides (conotoxins) with a diverse array of molecular targets, including voltage- and ligand-gated ion channels, G-protein coupled receptors and neurotransmitter transporters. Several conotoxins have found applications as research tools, with some being used or developed as therapeutics. The primary objective of this study was the large-scale discovery of conotoxin sequences from the venom gland of an Australian cone snail species, Conus victoriae. Using cDNA library normalization, high-throughput 454 sequencing, de novo transcriptome assembly and annotation with BLASTX and profile hidden Markov models, we discovered over 100 unique conotoxin sequences from 20 gene superfamilies, the highest diversity of conotoxins so far reported in a single study. Many of the sequences identified are new members of known conotoxin superfamilies, some help to redefine these superfamilies and others represent altogether new classes of conotoxins. In addition, we have demonstrated an efficient combination of methods to mine an animal venom gland and generate a library of sequences encoding bioactive peptides.
KW - Amino Acid Sequence
KW - Animals
KW - Conotoxins/genetics
KW - Conus Snail/genetics
KW - DNA, Complementary/genetics
KW - Molecular Sequence Annotation
KW - Molecular Sequence Data
KW - Sequence Analysis, Protein
U2 - 10.1371/journal.pone.0087648
DO - 10.1371/journal.pone.0087648
M3 - Journal article
C2 - 24505301
VL - 9
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 2
M1 - e87648
ER -
ID: 232824569