Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets

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Standard

Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets. / Nielsen, Jens Høiriis.

I: Biomedica Biochimica Acta, Bind 44, Nr. 1, 1985, s. 161-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Nielsen, JH 1985, 'Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets', Biomedica Biochimica Acta, bind 44, nr. 1, s. 161-6.

APA

Nielsen, J. H. (1985). Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets. Biomedica Biochimica Acta, 44(1), 161-6.

Vancouver

Nielsen JH. Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets. Biomedica Biochimica Acta. 1985;44(1):161-6.

Author

Nielsen, Jens Høiriis. / Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets. I: Biomedica Biochimica Acta. 1985 ; Bind 44, Nr. 1. s. 161-6.

Bibtex

@article{c58583c6b0bc43a8a9397359a723ae18,
title = "Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets",
abstract = "Glucose has been suggested to be the most important stimulus for beta cell replication in vivo and in vitro. In order to study the relationship between insulin secretion and DNA synthesis, newborn rat islets were cultured in the presence of different concentrations of glucose, theophylline and 3-isobutyl-1-methylxanthine (IBMX). DNA synthesis was evaluated by the incorporation of [3H]thymidine (3H-Tdr) into islet DNA, and the release of insulin and the content of insulin and DNA in the islets were determined. No difference in 3H-Tdr incorporation was observed after 24 h culture in 5.5, 11 and 22 mM glucose in spite of a dose dependent increase in insulin release. 5 mM theophylline potentiated the glucose induced insulin release but lowered both 3H-Tdr synthesis and insulin content in the islets. In contrast, 0.05 mM IBMX induced a significant stimulation of both insulin release and 3H-Tdr incorporation. However, long-term exposure to IBMX did not result in increased DNA content of the islets. Inhibition of the DNA synthesis by 5 mM hydroxyurea resulted in a marked reduction in DNA content of the islets but no decrease in either insulin release or insulin content when expressed per ng DNA. These results indicate that insulin secretion and DNA synthesis can be dissociated and may suggest participation of factors other than glucose in the control of beta cell replication.",
keywords = "Animals, Animals, Newborn, Cells, Cultured, DNA, Insulin, Islets of Langerhans, Rats",
author = "Nielsen, {Jens H{\o}iriis}",
year = "1985",
language = "English",
volume = "44",
pages = "161--6",
journal = "Biomedica Biochimica Acta",
issn = "0232-766X",
publisher = "Akademie Verlag GMBH",
number = "1",

}

RIS

TY - JOUR

T1 - Dissociation between insulin secretion and DNA synthesis in cultured pancreatic islets

AU - Nielsen, Jens Høiriis

PY - 1985

Y1 - 1985

N2 - Glucose has been suggested to be the most important stimulus for beta cell replication in vivo and in vitro. In order to study the relationship between insulin secretion and DNA synthesis, newborn rat islets were cultured in the presence of different concentrations of glucose, theophylline and 3-isobutyl-1-methylxanthine (IBMX). DNA synthesis was evaluated by the incorporation of [3H]thymidine (3H-Tdr) into islet DNA, and the release of insulin and the content of insulin and DNA in the islets were determined. No difference in 3H-Tdr incorporation was observed after 24 h culture in 5.5, 11 and 22 mM glucose in spite of a dose dependent increase in insulin release. 5 mM theophylline potentiated the glucose induced insulin release but lowered both 3H-Tdr synthesis and insulin content in the islets. In contrast, 0.05 mM IBMX induced a significant stimulation of both insulin release and 3H-Tdr incorporation. However, long-term exposure to IBMX did not result in increased DNA content of the islets. Inhibition of the DNA synthesis by 5 mM hydroxyurea resulted in a marked reduction in DNA content of the islets but no decrease in either insulin release or insulin content when expressed per ng DNA. These results indicate that insulin secretion and DNA synthesis can be dissociated and may suggest participation of factors other than glucose in the control of beta cell replication.

AB - Glucose has been suggested to be the most important stimulus for beta cell replication in vivo and in vitro. In order to study the relationship between insulin secretion and DNA synthesis, newborn rat islets were cultured in the presence of different concentrations of glucose, theophylline and 3-isobutyl-1-methylxanthine (IBMX). DNA synthesis was evaluated by the incorporation of [3H]thymidine (3H-Tdr) into islet DNA, and the release of insulin and the content of insulin and DNA in the islets were determined. No difference in 3H-Tdr incorporation was observed after 24 h culture in 5.5, 11 and 22 mM glucose in spite of a dose dependent increase in insulin release. 5 mM theophylline potentiated the glucose induced insulin release but lowered both 3H-Tdr synthesis and insulin content in the islets. In contrast, 0.05 mM IBMX induced a significant stimulation of both insulin release and 3H-Tdr incorporation. However, long-term exposure to IBMX did not result in increased DNA content of the islets. Inhibition of the DNA synthesis by 5 mM hydroxyurea resulted in a marked reduction in DNA content of the islets but no decrease in either insulin release or insulin content when expressed per ng DNA. These results indicate that insulin secretion and DNA synthesis can be dissociated and may suggest participation of factors other than glucose in the control of beta cell replication.

KW - Animals

KW - Animals, Newborn

KW - Cells, Cultured

KW - DNA

KW - Insulin

KW - Islets of Langerhans

KW - Rats

M3 - Journal article

VL - 44

SP - 161

EP - 166

JO - Biomedica Biochimica Acta

JF - Biomedica Biochimica Acta

SN - 0232-766X

IS - 1

ER -

ID: 47975197