Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans. / Bendtzen, K; Mandrup-Poulsen, T; Nerup, J; Dinarello, C A; Svenson, M; Nielsen, Jens Høiriis.

I: Science (New York, N.Y.), Bind 232, Nr. 4757, 20.06.1986, s. 1545-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bendtzen, K, Mandrup-Poulsen, T, Nerup, J, Dinarello, CA, Svenson, M & Nielsen, JH 1986, 'Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans', Science (New York, N.Y.), bind 232, nr. 4757, s. 1545-7.

APA

Bendtzen, K., Mandrup-Poulsen, T., Nerup, J., Dinarello, C. A., Svenson, M., & Nielsen, J. H. (1986). Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans. Science (New York, N.Y.), 232(4757), 1545-7.

Vancouver

Bendtzen K, Mandrup-Poulsen T, Nerup J, Dinarello CA, Svenson M, Nielsen JH. Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans. Science (New York, N.Y.). 1986 jun 20;232(4757):1545-7.

Author

Bendtzen, K ; Mandrup-Poulsen, T ; Nerup, J ; Dinarello, C A ; Svenson, M ; Nielsen, Jens Høiriis. / Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans. I: Science (New York, N.Y.). 1986 ; Bind 232, Nr. 4757. s. 1545-7.

Bibtex

@article{232a980ac5f147f9ba97929bf51bda99,
title = "Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans",
abstract = "Activated mononuclear cells appear to be important effector cells in autoimmune beta cell destruction leading to insulin-dependent (type 1) diabetes mellitus. Conditioned medium from activated mononuclear cells (from human blood) is cytotoxic to isolated rat and human islets of Langerhans. This cytotoxic activity was eliminated from crude cytokine preparations by adsorption with immobilized, purified antibody to interleukin-1 (IL-1). The islet-inhibitory activity and the IL-1 activity (determined by its comitogenic effect on thymocytes) were recovered by acid wash. Purified natural IL-1 and recombinant IL-1 derived from the predominant pI 7 form of human IL-1, consistently inhibited the insulin response. The pI 6 and pI 5 forms of natural IL-1 were ineffective. Natural and recombinant IL-1 exhibited similar dose responses in their islet-inhibitory effect and their thymocyte-stimulatory activity. Concentrations of IL-1 that inhibited islet activity were in the picomolar range. Hence, monocyte-derived pI 7 IL-1 may contribute to islet cell damage and therefore to the development of insulin-dependent diabetes mellitus.",
keywords = "Animals, Cell Survival, Glucagon, Humans, Insulin, Interferon-gamma, Interleukin-1, Islets of Langerhans, Monocytes, Rats, Recombinant Proteins, T-Lymphocytes",
author = "K Bendtzen and T Mandrup-Poulsen and J Nerup and Dinarello, {C A} and M Svenson and Nielsen, {Jens H{\o}iriis}",
year = "1986",
month = jun,
day = "20",
language = "English",
volume = "232",
pages = "1545--7",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4757",

}

RIS

TY - JOUR

T1 - Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans

AU - Bendtzen, K

AU - Mandrup-Poulsen, T

AU - Nerup, J

AU - Dinarello, C A

AU - Svenson, M

AU - Nielsen, Jens Høiriis

PY - 1986/6/20

Y1 - 1986/6/20

N2 - Activated mononuclear cells appear to be important effector cells in autoimmune beta cell destruction leading to insulin-dependent (type 1) diabetes mellitus. Conditioned medium from activated mononuclear cells (from human blood) is cytotoxic to isolated rat and human islets of Langerhans. This cytotoxic activity was eliminated from crude cytokine preparations by adsorption with immobilized, purified antibody to interleukin-1 (IL-1). The islet-inhibitory activity and the IL-1 activity (determined by its comitogenic effect on thymocytes) were recovered by acid wash. Purified natural IL-1 and recombinant IL-1 derived from the predominant pI 7 form of human IL-1, consistently inhibited the insulin response. The pI 6 and pI 5 forms of natural IL-1 were ineffective. Natural and recombinant IL-1 exhibited similar dose responses in their islet-inhibitory effect and their thymocyte-stimulatory activity. Concentrations of IL-1 that inhibited islet activity were in the picomolar range. Hence, monocyte-derived pI 7 IL-1 may contribute to islet cell damage and therefore to the development of insulin-dependent diabetes mellitus.

AB - Activated mononuclear cells appear to be important effector cells in autoimmune beta cell destruction leading to insulin-dependent (type 1) diabetes mellitus. Conditioned medium from activated mononuclear cells (from human blood) is cytotoxic to isolated rat and human islets of Langerhans. This cytotoxic activity was eliminated from crude cytokine preparations by adsorption with immobilized, purified antibody to interleukin-1 (IL-1). The islet-inhibitory activity and the IL-1 activity (determined by its comitogenic effect on thymocytes) were recovered by acid wash. Purified natural IL-1 and recombinant IL-1 derived from the predominant pI 7 form of human IL-1, consistently inhibited the insulin response. The pI 6 and pI 5 forms of natural IL-1 were ineffective. Natural and recombinant IL-1 exhibited similar dose responses in their islet-inhibitory effect and their thymocyte-stimulatory activity. Concentrations of IL-1 that inhibited islet activity were in the picomolar range. Hence, monocyte-derived pI 7 IL-1 may contribute to islet cell damage and therefore to the development of insulin-dependent diabetes mellitus.

KW - Animals

KW - Cell Survival

KW - Glucagon

KW - Humans

KW - Insulin

KW - Interferon-gamma

KW - Interleukin-1

KW - Islets of Langerhans

KW - Monocytes

KW - Rats

KW - Recombinant Proteins

KW - T-Lymphocytes

M3 - Journal article

C2 - 3086977

VL - 232

SP - 1545

EP - 1547

JO - Science

JF - Science

SN - 0036-8075

IS - 4757

ER -

ID: 47975012