Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice

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Standard

Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice. / Yassin, Mohammad; Kissow, Hannelouise; Vainer, Ben; Joseph, Philomeena Daphne; Hay-Schmidt, Anders; Olsen, Jørgen; Pedersen, Anders Elm.

I: Scientific Reports, Bind 8, 6905, 2018, s. 1-16.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Yassin, M, Kissow, H, Vainer, B, Joseph, PD, Hay-Schmidt, A, Olsen, J & Pedersen, AE 2018, 'Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice', Scientific Reports, bind 8, 6905, s. 1-16. https://doi.org/10.1038/s41598-018-24728-x

APA

Yassin, M., Kissow, H., Vainer, B., Joseph, P. D., Hay-Schmidt, A., Olsen, J., & Pedersen, A. E. (2018). Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice. Scientific Reports, 8, 1-16. [6905]. https://doi.org/10.1038/s41598-018-24728-x

Vancouver

Yassin M, Kissow H, Vainer B, Joseph PD, Hay-Schmidt A, Olsen J o.a. Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice. Scientific Reports. 2018;8:1-16. 6905. https://doi.org/10.1038/s41598-018-24728-x

Author

Yassin, Mohammad ; Kissow, Hannelouise ; Vainer, Ben ; Joseph, Philomeena Daphne ; Hay-Schmidt, Anders ; Olsen, Jørgen ; Pedersen, Anders Elm. / Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice. I: Scientific Reports. 2018 ; Bind 8. s. 1-16.

Bibtex

@article{ba9468009e3b4211aa8e68d7a410f92e,
title = "Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice",
abstract = "Cytoglobin (Cygb) is a member of the hemoglobin family and is thought to protect against cellular hypoxia and oxidative stress. These functions may be particularly important in inflammation-induced cancer, e.g., in patients with ulcerative colitis (UC). In this study, we investigated the development of inflammation and tumors in a murine model of inflammation-induced colorectal cancer using a combined treatment of azoxymethane and dextran sulfate sodium. A bioinformatics analysis of genome-wide expression data revealed increased colonic inflammation at the molecular level accompanied by enhanced macroscopic tumor development in Cygb-deficient mice. Moreover, the expression of the UC-associated gene neurexophilin and PC-esterase domain family member 4 (Nxpe4) depended on the presence of Cygb in the inflamed colonic mucosa. Compared to wild type mice, RT-qPCR confirmed a 14-fold (p = 0.0003) decrease in Nxpe4 expression in the inflamed colonic mucosa from Cygb-deficient mice. An analysis of Cygb protein expression suggested that Cygb is expressed in fibroblast-like cells surrounding the colonic crypts. Histological examinations of early induced lesions suggested that the effect of Cygb is primarily at the level of tumor promotion. In conclusion, in this model, Cygb primarily seemed to inhibit the development of established microadenomas.",
author = "Mohammad Yassin and Hannelouise Kissow and Ben Vainer and Joseph, {Philomeena Daphne} and Anders Hay-Schmidt and J{\o}rgen Olsen and Pedersen, {Anders Elm}",
year = "2018",
doi = "10.1038/s41598-018-24728-x",
language = "English",
volume = "8",
pages = "1--16",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice

AU - Yassin, Mohammad

AU - Kissow, Hannelouise

AU - Vainer, Ben

AU - Joseph, Philomeena Daphne

AU - Hay-Schmidt, Anders

AU - Olsen, Jørgen

AU - Pedersen, Anders Elm

PY - 2018

Y1 - 2018

N2 - Cytoglobin (Cygb) is a member of the hemoglobin family and is thought to protect against cellular hypoxia and oxidative stress. These functions may be particularly important in inflammation-induced cancer, e.g., in patients with ulcerative colitis (UC). In this study, we investigated the development of inflammation and tumors in a murine model of inflammation-induced colorectal cancer using a combined treatment of azoxymethane and dextran sulfate sodium. A bioinformatics analysis of genome-wide expression data revealed increased colonic inflammation at the molecular level accompanied by enhanced macroscopic tumor development in Cygb-deficient mice. Moreover, the expression of the UC-associated gene neurexophilin and PC-esterase domain family member 4 (Nxpe4) depended on the presence of Cygb in the inflamed colonic mucosa. Compared to wild type mice, RT-qPCR confirmed a 14-fold (p = 0.0003) decrease in Nxpe4 expression in the inflamed colonic mucosa from Cygb-deficient mice. An analysis of Cygb protein expression suggested that Cygb is expressed in fibroblast-like cells surrounding the colonic crypts. Histological examinations of early induced lesions suggested that the effect of Cygb is primarily at the level of tumor promotion. In conclusion, in this model, Cygb primarily seemed to inhibit the development of established microadenomas.

AB - Cytoglobin (Cygb) is a member of the hemoglobin family and is thought to protect against cellular hypoxia and oxidative stress. These functions may be particularly important in inflammation-induced cancer, e.g., in patients with ulcerative colitis (UC). In this study, we investigated the development of inflammation and tumors in a murine model of inflammation-induced colorectal cancer using a combined treatment of azoxymethane and dextran sulfate sodium. A bioinformatics analysis of genome-wide expression data revealed increased colonic inflammation at the molecular level accompanied by enhanced macroscopic tumor development in Cygb-deficient mice. Moreover, the expression of the UC-associated gene neurexophilin and PC-esterase domain family member 4 (Nxpe4) depended on the presence of Cygb in the inflamed colonic mucosa. Compared to wild type mice, RT-qPCR confirmed a 14-fold (p = 0.0003) decrease in Nxpe4 expression in the inflamed colonic mucosa from Cygb-deficient mice. An analysis of Cygb protein expression suggested that Cygb is expressed in fibroblast-like cells surrounding the colonic crypts. Histological examinations of early induced lesions suggested that the effect of Cygb is primarily at the level of tumor promotion. In conclusion, in this model, Cygb primarily seemed to inhibit the development of established microadenomas.

U2 - 10.1038/s41598-018-24728-x

DO - 10.1038/s41598-018-24728-x

M3 - Journal article

VL - 8

SP - 1

EP - 16

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 6905

ER -

ID: 196438637