Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets

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Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets. / Smith, Julie; Christoffersen, Christina; Nørgaard, Linn Maiken; Olsen, Lisbeth Høier; Vejlstrup, Niels G.; Andersen, Claus Bøgelund; Gøtze, Jens P.

I: Endocrinology, Bind 154, Nr. 5, 2013, s. 1864-1872.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Smith, J, Christoffersen, C, Nørgaard, LM, Olsen, LH, Vejlstrup, NG, Andersen, CB & Gøtze, JP 2013, 'Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets', Endocrinology, bind 154, nr. 5, s. 1864-1872. https://doi.org/10.1210/en.2012-2186

APA

Smith, J., Christoffersen, C., Nørgaard, L. M., Olsen, L. H., Vejlstrup, N. G., Andersen, C. B., & Gøtze, J. P. (2013). Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets. Endocrinology, 154(5), 1864-1872. https://doi.org/10.1210/en.2012-2186

Vancouver

Smith J, Christoffersen C, Nørgaard LM, Olsen LH, Vejlstrup NG, Andersen CB o.a. Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets. Endocrinology. 2013;154(5):1864-1872. https://doi.org/10.1210/en.2012-2186

Author

Smith, Julie ; Christoffersen, Christina ; Nørgaard, Linn Maiken ; Olsen, Lisbeth Høier ; Vejlstrup, Niels G. ; Andersen, Claus Bøgelund ; Gøtze, Jens P. / Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets. I: Endocrinology. 2013 ; Bind 154, Nr. 5. s. 1864-1872.

Bibtex

@article{6d44fb7ac04d42e29014cdbf3302bc88,
title = "Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets",
abstract = "Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.",
keywords = "Animals, Animals, Newborn, Atrial Natriuretic Factor, Coronary Circulation, Echocardiography, Female, Fetal Blood, Gene Expression, Male, Myocardium, Natriuretic Peptide, Brain, Osmolar Concentration, Pregnancy, Swine, Time Factors",
author = "Julie Smith and Christina Christoffersen and N{\o}rgaard, {Linn Maiken} and Olsen, {Lisbeth H{\o}ier} and Vejlstrup, {Niels G.} and Andersen, {Claus B{\o}gelund} and G{\o}tze, {Jens P.}",
year = "2013",
doi = "10.1210/en.2012-2186",
language = "English",
volume = "154",
pages = "1864--1872",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets

AU - Smith, Julie

AU - Christoffersen, Christina

AU - Nørgaard, Linn Maiken

AU - Olsen, Lisbeth Høier

AU - Vejlstrup, Niels G.

AU - Andersen, Claus Bøgelund

AU - Gøtze, Jens P.

PY - 2013

Y1 - 2013

N2 - Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.

AB - Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.

KW - Animals

KW - Animals, Newborn

KW - Atrial Natriuretic Factor

KW - Coronary Circulation

KW - Echocardiography

KW - Female

KW - Fetal Blood

KW - Gene Expression

KW - Male

KW - Myocardium

KW - Natriuretic Peptide, Brain

KW - Osmolar Concentration

KW - Pregnancy

KW - Swine

KW - Time Factors

U2 - 10.1210/en.2012-2186

DO - 10.1210/en.2012-2186

M3 - Journal article

C2 - 23539511

VL - 154

SP - 1864

EP - 1872

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -

ID: 113092981