Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression: implications for atherosclerosis research

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Standard

Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression : implications for atherosclerosis research. / Bisgaard, Line S; Mogensen, Christina K; Rosendahl, Alexander; Cucak, Helena; Nielsen, Lars Bo; Rasmussen, Salka E; Pedersen, Tanja X.

I: Scientific Reports, Bind 6, 35234, 13.10.2016.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bisgaard, LS, Mogensen, CK, Rosendahl, A, Cucak, H, Nielsen, LB, Rasmussen, SE & Pedersen, TX 2016, 'Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression: implications for atherosclerosis research' Scientific Reports, bind 6, 35234. https://doi.org/10.1038/srep35234

APA

Bisgaard, L. S., Mogensen, C. K., Rosendahl, A., Cucak, H., Nielsen, L. B., Rasmussen, S. E., & Pedersen, T. X. (2016). Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression: implications for atherosclerosis research. Scientific Reports, 6, [35234]. https://doi.org/10.1038/srep35234

Vancouver

Bisgaard LS, Mogensen CK, Rosendahl A, Cucak H, Nielsen LB, Rasmussen SE o.a. Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression: implications for atherosclerosis research. Scientific Reports. 2016 okt 13;6. 35234. https://doi.org/10.1038/srep35234

Author

Bisgaard, Line S ; Mogensen, Christina K ; Rosendahl, Alexander ; Cucak, Helena ; Nielsen, Lars Bo ; Rasmussen, Salka E ; Pedersen, Tanja X. / Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression : implications for atherosclerosis research. I: Scientific Reports. 2016 ; Bind 6.

Bibtex

@article{84b23a0594914d6caa46104b6645a3aa,
title = "Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression: implications for atherosclerosis research",
abstract = "Macrophages are heterogeneous and can polarize into specific subsets, e.g. pro-inflammatory M1-like and re-modelling M2-like macrophages. To determine if peritoneal macrophages (PEMs) or bone marrow derived macrophages (BMDMs) resembled aortic macrophages from ApoE-/- mice, their M1/M2 phenotype, inflammatory status, and lipid metabolism signatures were compared. oxLDL accumulation was similar in PEMs and BMDMs. On protein expression level, BMDMs showed an M2-like CD206(high)CD11c(low) profile, while cholesterol loading led to enhanced CD11c expression and reduced MCP-1 secretion. In contrast, PEMs expressed low levels of CD206 and CD11c, and responded to cholesterol loading by increasing CD11c expression and MCP-1 secretion. mRNA expression of M1/M2 markers was higher in PEMS than BMDMs, while lipid metabolism genes were similarly expressed. Whole aorta flow cytometry showed an accumulation of M2-like CD206(high)CD11c(low) macrophages in advanced versus early atherosclerotic disease in ApoE-/- mice. In isolated lesions, mRNA levels of the M2 markers Socs2, CD206, Retnla, and IL4 were downregulated with increasing disease severity. Likewise, mRNA expression of lipid metabolism genes (SREBP2, ACSL1, SRB1, DGAT1, and cpt1a) was decreased in advanced versus early lesions. In conclusion, PEMs and BMDMs are phenotypically distinct and differ from macrophages in lesions with respect to expression of M1/M2 markers and lipid metabolism genes.",
author = "Bisgaard, {Line S} and Mogensen, {Christina K} and Alexander Rosendahl and Helena Cucak and Nielsen, {Lars Bo} and Rasmussen, {Salka E} and Pedersen, {Tanja X}",
year = "2016",
month = "10",
day = "13",
doi = "10.1038/srep35234",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression

T2 - implications for atherosclerosis research

AU - Bisgaard, Line S

AU - Mogensen, Christina K

AU - Rosendahl, Alexander

AU - Cucak, Helena

AU - Nielsen, Lars Bo

AU - Rasmussen, Salka E

AU - Pedersen, Tanja X

PY - 2016/10/13

Y1 - 2016/10/13

N2 - Macrophages are heterogeneous and can polarize into specific subsets, e.g. pro-inflammatory M1-like and re-modelling M2-like macrophages. To determine if peritoneal macrophages (PEMs) or bone marrow derived macrophages (BMDMs) resembled aortic macrophages from ApoE-/- mice, their M1/M2 phenotype, inflammatory status, and lipid metabolism signatures were compared. oxLDL accumulation was similar in PEMs and BMDMs. On protein expression level, BMDMs showed an M2-like CD206(high)CD11c(low) profile, while cholesterol loading led to enhanced CD11c expression and reduced MCP-1 secretion. In contrast, PEMs expressed low levels of CD206 and CD11c, and responded to cholesterol loading by increasing CD11c expression and MCP-1 secretion. mRNA expression of M1/M2 markers was higher in PEMS than BMDMs, while lipid metabolism genes were similarly expressed. Whole aorta flow cytometry showed an accumulation of M2-like CD206(high)CD11c(low) macrophages in advanced versus early atherosclerotic disease in ApoE-/- mice. In isolated lesions, mRNA levels of the M2 markers Socs2, CD206, Retnla, and IL4 were downregulated with increasing disease severity. Likewise, mRNA expression of lipid metabolism genes (SREBP2, ACSL1, SRB1, DGAT1, and cpt1a) was decreased in advanced versus early lesions. In conclusion, PEMs and BMDMs are phenotypically distinct and differ from macrophages in lesions with respect to expression of M1/M2 markers and lipid metabolism genes.

AB - Macrophages are heterogeneous and can polarize into specific subsets, e.g. pro-inflammatory M1-like and re-modelling M2-like macrophages. To determine if peritoneal macrophages (PEMs) or bone marrow derived macrophages (BMDMs) resembled aortic macrophages from ApoE-/- mice, their M1/M2 phenotype, inflammatory status, and lipid metabolism signatures were compared. oxLDL accumulation was similar in PEMs and BMDMs. On protein expression level, BMDMs showed an M2-like CD206(high)CD11c(low) profile, while cholesterol loading led to enhanced CD11c expression and reduced MCP-1 secretion. In contrast, PEMs expressed low levels of CD206 and CD11c, and responded to cholesterol loading by increasing CD11c expression and MCP-1 secretion. mRNA expression of M1/M2 markers was higher in PEMS than BMDMs, while lipid metabolism genes were similarly expressed. Whole aorta flow cytometry showed an accumulation of M2-like CD206(high)CD11c(low) macrophages in advanced versus early atherosclerotic disease in ApoE-/- mice. In isolated lesions, mRNA levels of the M2 markers Socs2, CD206, Retnla, and IL4 were downregulated with increasing disease severity. Likewise, mRNA expression of lipid metabolism genes (SREBP2, ACSL1, SRB1, DGAT1, and cpt1a) was decreased in advanced versus early lesions. In conclusion, PEMs and BMDMs are phenotypically distinct and differ from macrophages in lesions with respect to expression of M1/M2 markers and lipid metabolism genes.

U2 - 10.1038/srep35234

DO - 10.1038/srep35234

M3 - Journal article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 35234

ER -

ID: 167473847