Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. / Larsen, Steen; Nielsen, Joachim; Neigaard Nielsen, Christina; Nielsen, Lars Bo; Wibrand, Flemming; Stride, Nis; Schroder, Henrik Daa; Boushel, Robert; Helge, Jørn W; Dela, Flemming; Hey-Mogensen, Martin.

I: Journal of Physiology, Bind 590, 2012, s. 3349-3360.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Larsen, S, Nielsen, J, Neigaard Nielsen, C, Nielsen, LB, Wibrand, F, Stride, N, Schroder, HD, Boushel, R, Helge, JW, Dela, F & Hey-Mogensen, M 2012, 'Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects', Journal of Physiology, bind 590, s. 3349-3360. https://doi.org/10.1113/jphysiol.2012.230185

APA

Larsen, S., Nielsen, J., Neigaard Nielsen, C., Nielsen, L. B., Wibrand, F., Stride, N., ... Hey-Mogensen, M. (2012). Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. Journal of Physiology, 590, 3349-3360. https://doi.org/10.1113/jphysiol.2012.230185

Vancouver

Larsen S, Nielsen J, Neigaard Nielsen C, Nielsen LB, Wibrand F, Stride N o.a. Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. Journal of Physiology. 2012;590:3349-3360. https://doi.org/10.1113/jphysiol.2012.230185

Author

Larsen, Steen ; Nielsen, Joachim ; Neigaard Nielsen, Christina ; Nielsen, Lars Bo ; Wibrand, Flemming ; Stride, Nis ; Schroder, Henrik Daa ; Boushel, Robert ; Helge, Jørn W ; Dela, Flemming ; Hey-Mogensen, Martin. / Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. I: Journal of Physiology. 2012 ; Bind 590. s. 3349-3360.

Bibtex

@article{8c74ae6bb85d40969f072f96b9eb0548,
title = "Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects",
abstract = "Skeletal muscle mitochondrial content varies extensively between human subjects. Biochemical measures of mitochondrial proteins, enzyme activities and lipids are often used as markers of mitochondrial content and muscle oxidative capacity (OXPHOS). The purpose of this study was to determine how closely associated these commonly used biochemical measures are to muscle mitochondrial content and muscle oxidative capacity (OXPHOS).Sixteen young healthy male subjects were recruited for this study. Subjects completed a graded exercise test to determine maximal oxygen uptake (VO(2peak)) and muscle biopsies were obtained from the vastus lateralis. Mitochondrial content was determined using transmission electron microscopy imaging and OXPHOS was determined as the maximal coupled respiration in permeabilized fibers. Biomarkers of interest were citrate synthase (CS) activity, cardiolipin content, mitochondrial DNA content, complex I-V protein content, and complex I-IV activity. Spearman correlation coefficient tests and Lin's concordance tests were applied to assess the absolute and relative association between the markers and mitochondrial content or OXPHOS.Subjects had a large range in VO(2peak) (range 29.9 -71.6 ml/min/kg) and in mitochondrial content (4-15{\%} of cell volume). Cardiolipin content showed the strongest association to mitochondrial content followed by CS- and complex I activity. mtDNA was not related to mitochondrial content. Complex IV activity showed the strongest association to muscle oxidative capacity followed by complex II activity.We conclude that cardiolipin content, CS and complex I activity are the biomarkers that exhibit the strongest association to mitochondrial content, while complex IV activity is strongly associated with OXPHOS capacity in human skeletal muscle.",
author = "Steen Larsen and Joachim Nielsen and {Neigaard Nielsen}, Christina and Nielsen, {Lars Bo} and Flemming Wibrand and Nis Stride and Schroder, {Henrik Daa} and Robert Boushel and Helge, {J{\o}rn W} and Flemming Dela and Martin Hey-Mogensen",
year = "2012",
doi = "10.1113/jphysiol.2012.230185",
language = "English",
volume = "590",
pages = "3349--3360",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects

AU - Larsen, Steen

AU - Nielsen, Joachim

AU - Neigaard Nielsen, Christina

AU - Nielsen, Lars Bo

AU - Wibrand, Flemming

AU - Stride, Nis

AU - Schroder, Henrik Daa

AU - Boushel, Robert

AU - Helge, Jørn W

AU - Dela, Flemming

AU - Hey-Mogensen, Martin

PY - 2012

Y1 - 2012

N2 - Skeletal muscle mitochondrial content varies extensively between human subjects. Biochemical measures of mitochondrial proteins, enzyme activities and lipids are often used as markers of mitochondrial content and muscle oxidative capacity (OXPHOS). The purpose of this study was to determine how closely associated these commonly used biochemical measures are to muscle mitochondrial content and muscle oxidative capacity (OXPHOS).Sixteen young healthy male subjects were recruited for this study. Subjects completed a graded exercise test to determine maximal oxygen uptake (VO(2peak)) and muscle biopsies were obtained from the vastus lateralis. Mitochondrial content was determined using transmission electron microscopy imaging and OXPHOS was determined as the maximal coupled respiration in permeabilized fibers. Biomarkers of interest were citrate synthase (CS) activity, cardiolipin content, mitochondrial DNA content, complex I-V protein content, and complex I-IV activity. Spearman correlation coefficient tests and Lin's concordance tests were applied to assess the absolute and relative association between the markers and mitochondrial content or OXPHOS.Subjects had a large range in VO(2peak) (range 29.9 -71.6 ml/min/kg) and in mitochondrial content (4-15% of cell volume). Cardiolipin content showed the strongest association to mitochondrial content followed by CS- and complex I activity. mtDNA was not related to mitochondrial content. Complex IV activity showed the strongest association to muscle oxidative capacity followed by complex II activity.We conclude that cardiolipin content, CS and complex I activity are the biomarkers that exhibit the strongest association to mitochondrial content, while complex IV activity is strongly associated with OXPHOS capacity in human skeletal muscle.

AB - Skeletal muscle mitochondrial content varies extensively between human subjects. Biochemical measures of mitochondrial proteins, enzyme activities and lipids are often used as markers of mitochondrial content and muscle oxidative capacity (OXPHOS). The purpose of this study was to determine how closely associated these commonly used biochemical measures are to muscle mitochondrial content and muscle oxidative capacity (OXPHOS).Sixteen young healthy male subjects were recruited for this study. Subjects completed a graded exercise test to determine maximal oxygen uptake (VO(2peak)) and muscle biopsies were obtained from the vastus lateralis. Mitochondrial content was determined using transmission electron microscopy imaging and OXPHOS was determined as the maximal coupled respiration in permeabilized fibers. Biomarkers of interest were citrate synthase (CS) activity, cardiolipin content, mitochondrial DNA content, complex I-V protein content, and complex I-IV activity. Spearman correlation coefficient tests and Lin's concordance tests were applied to assess the absolute and relative association between the markers and mitochondrial content or OXPHOS.Subjects had a large range in VO(2peak) (range 29.9 -71.6 ml/min/kg) and in mitochondrial content (4-15% of cell volume). Cardiolipin content showed the strongest association to mitochondrial content followed by CS- and complex I activity. mtDNA was not related to mitochondrial content. Complex IV activity showed the strongest association to muscle oxidative capacity followed by complex II activity.We conclude that cardiolipin content, CS and complex I activity are the biomarkers that exhibit the strongest association to mitochondrial content, while complex IV activity is strongly associated with OXPHOS capacity in human skeletal muscle.

U2 - 10.1113/jphysiol.2012.230185

DO - 10.1113/jphysiol.2012.230185

M3 - Journal article

C2 - 22586215

VL - 590

SP - 3349

EP - 3360

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

ER -

ID: 38431761