Beta-cell function in isolated human pancreatic islets in long-term tissue culture

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Standard

Beta-cell function in isolated human pancreatic islets in long-term tissue culture. / Nielsen, Jens Høiriis.

I: Acta Biologica et Medica Germanica, Bind 40, Nr. 1, 1981, s. 55-60.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nielsen, JH 1981, 'Beta-cell function in isolated human pancreatic islets in long-term tissue culture', Acta Biologica et Medica Germanica, bind 40, nr. 1, s. 55-60.

APA

Nielsen, J. H. (1981). Beta-cell function in isolated human pancreatic islets in long-term tissue culture. Acta Biologica et Medica Germanica, 40(1), 55-60.

Vancouver

Nielsen JH. Beta-cell function in isolated human pancreatic islets in long-term tissue culture. Acta Biologica et Medica Germanica. 1981;40(1):55-60.

Author

Nielsen, Jens Høiriis. / Beta-cell function in isolated human pancreatic islets in long-term tissue culture. I: Acta Biologica et Medica Germanica. 1981 ; Bind 40, Nr. 1. s. 55-60.

Bibtex

@article{42276b5bf25d4e409cec225d6b24eafc,
title = "Beta-cell function in isolated human pancreatic islets in long-term tissue culture",
abstract = "Human pancreatic islets were isolated by collagenase treatment of pancreatic tissue obtained from 27 individuals aged 12 to 69 years. The islets were maintained free floating in tissue culture medium RPMI 1640 supplemented with calf or human serum. In two cases the insulin production was followed up to nearly two years. The insulin production rate of the individual islet preparations varied between 0.2 and 8 ng per islet per day. No significant correlation with donor age or sex was found. The glucose concentration in the medium influenced the insulin release in a dose dependent manner. The acute response of the cultured islets to glucose was evaluated both by batch incubation and by perifusion. Both in the acute and the chronic experiments maximal insulin release was found at 10 mM glucose. In conclusion, these experiments indicate that viable islets of Langerhans can be obtained from adult human pancreatic tissue and that their beta-cell function can be maintained for up to two years. The variation in insulin production rate could not be ascribed to age or sex and may reflect both physiological and methodological factors.",
keywords = "Adolescent, Adult, Aged, Cells, Cultured, Child, Glucose, Humans, Insulin, Islets of Langerhans, Microbial Collagenase, Middle Aged, Theophylline, Time Factors",
author = "Nielsen, {Jens H{\o}iriis}",
year = "1981",
language = "English",
volume = "40",
pages = "55--60",
journal = "Acta Biologica et Medica Germanica",
issn = "0001-5318",
publisher = "Akademie Verlag GMBH",
number = "1",

}

RIS

TY - JOUR

T1 - Beta-cell function in isolated human pancreatic islets in long-term tissue culture

AU - Nielsen, Jens Høiriis

PY - 1981

Y1 - 1981

N2 - Human pancreatic islets were isolated by collagenase treatment of pancreatic tissue obtained from 27 individuals aged 12 to 69 years. The islets were maintained free floating in tissue culture medium RPMI 1640 supplemented with calf or human serum. In two cases the insulin production was followed up to nearly two years. The insulin production rate of the individual islet preparations varied between 0.2 and 8 ng per islet per day. No significant correlation with donor age or sex was found. The glucose concentration in the medium influenced the insulin release in a dose dependent manner. The acute response of the cultured islets to glucose was evaluated both by batch incubation and by perifusion. Both in the acute and the chronic experiments maximal insulin release was found at 10 mM glucose. In conclusion, these experiments indicate that viable islets of Langerhans can be obtained from adult human pancreatic tissue and that their beta-cell function can be maintained for up to two years. The variation in insulin production rate could not be ascribed to age or sex and may reflect both physiological and methodological factors.

AB - Human pancreatic islets were isolated by collagenase treatment of pancreatic tissue obtained from 27 individuals aged 12 to 69 years. The islets were maintained free floating in tissue culture medium RPMI 1640 supplemented with calf or human serum. In two cases the insulin production was followed up to nearly two years. The insulin production rate of the individual islet preparations varied between 0.2 and 8 ng per islet per day. No significant correlation with donor age or sex was found. The glucose concentration in the medium influenced the insulin release in a dose dependent manner. The acute response of the cultured islets to glucose was evaluated both by batch incubation and by perifusion. Both in the acute and the chronic experiments maximal insulin release was found at 10 mM glucose. In conclusion, these experiments indicate that viable islets of Langerhans can be obtained from adult human pancreatic tissue and that their beta-cell function can be maintained for up to two years. The variation in insulin production rate could not be ascribed to age or sex and may reflect both physiological and methodological factors.

KW - Adolescent

KW - Adult

KW - Aged

KW - Cells, Cultured

KW - Child

KW - Glucose

KW - Humans

KW - Insulin

KW - Islets of Langerhans

KW - Microbial Collagenase

KW - Middle Aged

KW - Theophylline

KW - Time Factors

M3 - Journal article

C2 - 6266182

VL - 40

SP - 55

EP - 60

JO - Acta Biologica et Medica Germanica

JF - Acta Biologica et Medica Germanica

SN - 0001-5318

IS - 1

ER -

ID: 47975509