Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography

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Standard

Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography. / Linde, S; Welinder, B S; Nielsen, Jens Høiriis.

I: Journal of Chromatography A, Bind 614, Nr. 2, 05.05.1993, s. 185-204.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Linde, S, Welinder, BS & Nielsen, JH 1993, 'Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography', Journal of Chromatography A, bind 614, nr. 2, s. 185-204.

APA

Linde, S., Welinder, B. S., & Nielsen, J. H. (1993). Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography. Journal of Chromatography A, 614(2), 185-204.

Vancouver

Linde S, Welinder BS, Nielsen JH. Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography. Journal of Chromatography A. 1993 maj 5;614(2):185-204.

Author

Linde, S ; Welinder, B S ; Nielsen, Jens Høiriis. / Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography. I: Journal of Chromatography A. 1993 ; Bind 614, Nr. 2. s. 185-204.

Bibtex

@article{513bfa2c98894338a7f757e2062d016c,
title = "Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography",
abstract = "Proinsulin is synthesized in the beta-cells of the endocrine pancreas, one of the four cell types found in the islets of Langerhans. Specific enzymatic cleavage of proinsulin results in the formation of equimolar amounts of insulin and C-peptide, via several intermediate split-proinsulin forms. Most mammals produce a single insulin, but in rodents two non-allelic insulin genes are expressed. There is an inverse ratio between the two insulins in rats and mice, the reason for this being unknown. It has been suggested that differences in transcription, translation (biosynthesis) and/or posttranslational processes (enzymatic conversion, intracellular degradation) could be possible explanations. Elevated amounts of proinsulin-immunoreactive material (PIM) have been described to occur in various conditions/diseases, suggesting alterations in beta-cell function, but the composition of the secreted PIM (intact proinsulin or its intermediates) has been incompletely determined. Studies of the biosynthesis of proinsulins and their conversion with the purpose of revealing some of these points depend on accessible reversed-phase high-performance liquid chromatographic (RP-HPLC) analyses capable of separating all the relevant, closely related polypeptides involved. This review will deal with the optimization of the RP-HPLC separations as well as sample preparation and recovery. Applications of the selected methods in the study of proinsulin biosynthesis and its conversion will also be presented.",
keywords = "Animals, C-Peptide, Chromatography, High Pressure Liquid, Humans, Insulin, Islets of Langerhans, Mice, Proinsulin, Rats",
author = "S Linde and Welinder, {B S} and Nielsen, {Jens H{\o}iriis}",
year = "1993",
month = may,
day = "5",
language = "English",
volume = "614",
pages = "185--204",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Analysis of proinsulin and its conversion products by reversed-phase high-performance liquid chromatography

AU - Linde, S

AU - Welinder, B S

AU - Nielsen, Jens Høiriis

PY - 1993/5/5

Y1 - 1993/5/5

N2 - Proinsulin is synthesized in the beta-cells of the endocrine pancreas, one of the four cell types found in the islets of Langerhans. Specific enzymatic cleavage of proinsulin results in the formation of equimolar amounts of insulin and C-peptide, via several intermediate split-proinsulin forms. Most mammals produce a single insulin, but in rodents two non-allelic insulin genes are expressed. There is an inverse ratio between the two insulins in rats and mice, the reason for this being unknown. It has been suggested that differences in transcription, translation (biosynthesis) and/or posttranslational processes (enzymatic conversion, intracellular degradation) could be possible explanations. Elevated amounts of proinsulin-immunoreactive material (PIM) have been described to occur in various conditions/diseases, suggesting alterations in beta-cell function, but the composition of the secreted PIM (intact proinsulin or its intermediates) has been incompletely determined. Studies of the biosynthesis of proinsulins and their conversion with the purpose of revealing some of these points depend on accessible reversed-phase high-performance liquid chromatographic (RP-HPLC) analyses capable of separating all the relevant, closely related polypeptides involved. This review will deal with the optimization of the RP-HPLC separations as well as sample preparation and recovery. Applications of the selected methods in the study of proinsulin biosynthesis and its conversion will also be presented.

AB - Proinsulin is synthesized in the beta-cells of the endocrine pancreas, one of the four cell types found in the islets of Langerhans. Specific enzymatic cleavage of proinsulin results in the formation of equimolar amounts of insulin and C-peptide, via several intermediate split-proinsulin forms. Most mammals produce a single insulin, but in rodents two non-allelic insulin genes are expressed. There is an inverse ratio between the two insulins in rats and mice, the reason for this being unknown. It has been suggested that differences in transcription, translation (biosynthesis) and/or posttranslational processes (enzymatic conversion, intracellular degradation) could be possible explanations. Elevated amounts of proinsulin-immunoreactive material (PIM) have been described to occur in various conditions/diseases, suggesting alterations in beta-cell function, but the composition of the secreted PIM (intact proinsulin or its intermediates) has been incompletely determined. Studies of the biosynthesis of proinsulins and their conversion with the purpose of revealing some of these points depend on accessible reversed-phase high-performance liquid chromatographic (RP-HPLC) analyses capable of separating all the relevant, closely related polypeptides involved. This review will deal with the optimization of the RP-HPLC separations as well as sample preparation and recovery. Applications of the selected methods in the study of proinsulin biosynthesis and its conversion will also be presented.

KW - Animals

KW - C-Peptide

KW - Chromatography, High Pressure Liquid

KW - Humans

KW - Insulin

KW - Islets of Langerhans

KW - Mice

KW - Proinsulin

KW - Rats

M3 - Journal article

C2 - 8314931

VL - 614

SP - 185

EP - 204

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 2

ER -

ID: 47973405