An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine

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An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine. / Svendsen, Berit; Pedersen, Jens; Albrechtsen, Nicolai Jacob Wewer; Hartmann, Bolette; Toräng, Signe; Rehfeld, Jens F; Seier Poulsen, Steen; Holst, Jens Juul.

I: Endocrinology, Bind 156, Nr. 3, en20141710, 03.2015, s. 847-857.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Svendsen, B, Pedersen, J, Albrechtsen, NJW, Hartmann, B, Toräng, S, Rehfeld, JF, Seier Poulsen, S & Holst, JJ 2015, 'An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine', Endocrinology, bind 156, nr. 3, en20141710, s. 847-857. https://doi.org/10.1210/en.2014-1710

APA

Svendsen, B., Pedersen, J., Albrechtsen, N. J. W., Hartmann, B., Toräng, S., Rehfeld, J. F., ... Holst, J. J. (2015). An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine. Endocrinology, 156(3), 847-857. [en20141710]. https://doi.org/10.1210/en.2014-1710

Vancouver

Svendsen B, Pedersen J, Albrechtsen NJW, Hartmann B, Toräng S, Rehfeld JF o.a. An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine. Endocrinology. 2015 mar;156(3):847-857. en20141710. https://doi.org/10.1210/en.2014-1710

Author

Svendsen, Berit ; Pedersen, Jens ; Albrechtsen, Nicolai Jacob Wewer ; Hartmann, Bolette ; Toräng, Signe ; Rehfeld, Jens F ; Seier Poulsen, Steen ; Holst, Jens Juul. / An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine. I: Endocrinology. 2015 ; Bind 156, Nr. 3. s. 847-857.

Bibtex

@article{8bd990301c0044008262a00530ef7957,
title = "An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine",
abstract = "Gut endocrine cells are generally thought to have distinct localization and secretory products. Recent studies suggested that the cells are highly related and have potential to express more than one hormone. We studied co-expression and co-secretion of gut hormones in separate segments of rat small intestine. We measured secretion of glucagon-like peptide-1 (GLP-1), peptideYY (PYY), neurotensin, glucose-dependent insulinotropic polypeptide (GIP), and cholecystokinin (CCK) from proximal and distal half of the small intestine, isolated from male rats and perfused ex vivo. Hormone secretion was stimulated by bombesin, the phosphodiesterase inhibitor IBMX, and peptones. Furthermore, tissue samples collected along the intestine were analyzed for expression, hormone content, and cell densities including colocalization. Most hormones responded to all three stimuli (but no GIP response to bombesin). GLP-1 secretion was similar from proximal and distal intestine, whereas PYY was only secreted from the distal half. CCK and GIP were mainly secreted proximally, whereas neurotensin was equally secreted from both parts. Cell densities, hormone concentrations and expression patterns were generally parallel, with increasing values distally for GLP-1 and PYY, an exclusively proximal pattern for CCK, even distribution for neurotensin and GIP except for the most distal segments. PYY nearly always co-localized with GLP-1. About 20{\%} of GLP-1 cells co-localized with CCK and neurotensin, whereas GLP-1/GIP co-localization was rare. Our findings indicate that two L-cell types exist, a proximal one secreting GLP-1 (and possibly CCK and neurotensin), and a distal one secreting GLP-1 and PYY. GIP seems to be secreted from cells not co-secreting other peptides.",
author = "Berit Svendsen and Jens Pedersen and Albrechtsen, {Nicolai Jacob Wewer} and Bolette Hartmann and Signe Tor{\"a}ng and Rehfeld, {Jens F} and {Seier Poulsen}, Steen and Holst, {Jens Juul}",
year = "2015",
month = "3",
doi = "10.1210/en.2014-1710",
language = "English",
volume = "156",
pages = "847--857",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - An Analysis of Cosecretion and Coexpression of Gut Hormones From Male Rat Proximal and Distal Small Intestine

AU - Svendsen, Berit

AU - Pedersen, Jens

AU - Albrechtsen, Nicolai Jacob Wewer

AU - Hartmann, Bolette

AU - Toräng, Signe

AU - Rehfeld, Jens F

AU - Seier Poulsen, Steen

AU - Holst, Jens Juul

PY - 2015/3

Y1 - 2015/3

N2 - Gut endocrine cells are generally thought to have distinct localization and secretory products. Recent studies suggested that the cells are highly related and have potential to express more than one hormone. We studied co-expression and co-secretion of gut hormones in separate segments of rat small intestine. We measured secretion of glucagon-like peptide-1 (GLP-1), peptideYY (PYY), neurotensin, glucose-dependent insulinotropic polypeptide (GIP), and cholecystokinin (CCK) from proximal and distal half of the small intestine, isolated from male rats and perfused ex vivo. Hormone secretion was stimulated by bombesin, the phosphodiesterase inhibitor IBMX, and peptones. Furthermore, tissue samples collected along the intestine were analyzed for expression, hormone content, and cell densities including colocalization. Most hormones responded to all three stimuli (but no GIP response to bombesin). GLP-1 secretion was similar from proximal and distal intestine, whereas PYY was only secreted from the distal half. CCK and GIP were mainly secreted proximally, whereas neurotensin was equally secreted from both parts. Cell densities, hormone concentrations and expression patterns were generally parallel, with increasing values distally for GLP-1 and PYY, an exclusively proximal pattern for CCK, even distribution for neurotensin and GIP except for the most distal segments. PYY nearly always co-localized with GLP-1. About 20% of GLP-1 cells co-localized with CCK and neurotensin, whereas GLP-1/GIP co-localization was rare. Our findings indicate that two L-cell types exist, a proximal one secreting GLP-1 (and possibly CCK and neurotensin), and a distal one secreting GLP-1 and PYY. GIP seems to be secreted from cells not co-secreting other peptides.

AB - Gut endocrine cells are generally thought to have distinct localization and secretory products. Recent studies suggested that the cells are highly related and have potential to express more than one hormone. We studied co-expression and co-secretion of gut hormones in separate segments of rat small intestine. We measured secretion of glucagon-like peptide-1 (GLP-1), peptideYY (PYY), neurotensin, glucose-dependent insulinotropic polypeptide (GIP), and cholecystokinin (CCK) from proximal and distal half of the small intestine, isolated from male rats and perfused ex vivo. Hormone secretion was stimulated by bombesin, the phosphodiesterase inhibitor IBMX, and peptones. Furthermore, tissue samples collected along the intestine were analyzed for expression, hormone content, and cell densities including colocalization. Most hormones responded to all three stimuli (but no GIP response to bombesin). GLP-1 secretion was similar from proximal and distal intestine, whereas PYY was only secreted from the distal half. CCK and GIP were mainly secreted proximally, whereas neurotensin was equally secreted from both parts. Cell densities, hormone concentrations and expression patterns were generally parallel, with increasing values distally for GLP-1 and PYY, an exclusively proximal pattern for CCK, even distribution for neurotensin and GIP except for the most distal segments. PYY nearly always co-localized with GLP-1. About 20% of GLP-1 cells co-localized with CCK and neurotensin, whereas GLP-1/GIP co-localization was rare. Our findings indicate that two L-cell types exist, a proximal one secreting GLP-1 (and possibly CCK and neurotensin), and a distal one secreting GLP-1 and PYY. GIP seems to be secreted from cells not co-secreting other peptides.

U2 - 10.1210/en.2014-1710

DO - 10.1210/en.2014-1710

M3 - Journal article

C2 - 25535831

VL - 156

SP - 847

EP - 857

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

M1 - en20141710

ER -

ID: 129245044