Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle

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Standard

Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle. / Bechshøft, Cecilie J.L.; Jensen, Simon M.; Schjerling, Peter; Andersen, Jesper L.; Svensson, Rene B.; Eriksen, Christian S.; Mkumbuzi, Nonhlanhla S.; Kjaer, Michael; Mackey, Abigail L.

I: American Journal of Physiology: Cell Physiology, Bind 316, Nr. 6, 06.2019, s. C898-C912.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Bechshøft, CJL, Jensen, SM, Schjerling, P, Andersen, JL, Svensson, RB, Eriksen, CS, Mkumbuzi, NS, Kjaer, M & Mackey, AL 2019, 'Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle', American Journal of Physiology: Cell Physiology, bind 316, nr. 6, s. C898-C912. https://doi.org/10.1152/ajpcell.00049.2019

APA

Bechshøft, C. J. L., Jensen, S. M., Schjerling, P., Andersen, J. L., Svensson, R. B., Eriksen, C. S., Mkumbuzi, N. S., Kjaer, M., & Mackey, A. L. (2019). Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle. American Journal of Physiology: Cell Physiology, 316(6), C898-C912. https://doi.org/10.1152/ajpcell.00049.2019

Vancouver

Bechshøft CJL, Jensen SM, Schjerling P, Andersen JL, Svensson RB, Eriksen CS o.a. Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle. American Journal of Physiology: Cell Physiology. 2019 jun.;316(6):C898-C912. https://doi.org/10.1152/ajpcell.00049.2019

Author

Bechshøft, Cecilie J.L. ; Jensen, Simon M. ; Schjerling, Peter ; Andersen, Jesper L. ; Svensson, Rene B. ; Eriksen, Christian S. ; Mkumbuzi, Nonhlanhla S. ; Kjaer, Michael ; Mackey, Abigail L. / Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle. I: American Journal of Physiology: Cell Physiology. 2019 ; Bind 316, Nr. 6. s. C898-C912.

Bibtex

@article{4da7f9753b63420283c8754410952191,
title = "Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle",
abstract = "The decline in skeletal muscle regenerative capacity with age is partly attributed to muscle stem cell (satellite cell) dysfunction. Recent evidence has pointed to a strong interaction between myoblasts and fibroblasts, but the influence of age on this interaction is unknown. Additionally, while the native tissue environment is known to determine the properties of myogenic cells in vitro, how the ageing process alters this cell memory has not been established at the molecular level. We recruited 12 young and 12 elderly women, who performed a single bout of heavy resistance exercise with the knee extensor muscles of one leg. 5 days later, muscle biopsies were collected from both legs and myogenic cells and non-myogenic cells were isolated for in vitro experiments with mixed or separated cells, and analysed by immunostaining and RT-PCR. A lower myogenic fusion index was detected in the cells from the old vs. young women, in association with differences in gene expression levels of key myogenic regulatory factors and senescence, which were further altered by performing exercise prior to tissue sampling. Co-culture with non-myogenic cells from the elderly lead to a higher myogenic differentiation index compared to non-myogenic cells from the young. These findings show that the in vitro phenotype and molecular profile of human skeletal muscle myoblasts and fibroblasts is determined by the age and exercise state of the original in vivo environment and help explain how exercise can enhance muscle stem cell function in old age.",
author = "Bechsh{\o}ft, {Cecilie J.L.} and Jensen, {Simon M.} and Peter Schjerling and Andersen, {Jesper L.} and Svensson, {Rene B.} and Eriksen, {Christian S.} and Mkumbuzi, {Nonhlanhla S.} and Michael Kjaer and Mackey, {Abigail L.}",
year = "2019",
month = jun,
doi = "10.1152/ajpcell.00049.2019",
language = "English",
volume = "316",
pages = "C898--C912",
journal = "American Journal of Physiology: Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "6",

}

RIS

TY - JOUR

T1 - Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and non-myogenic cells derived from human skeletal muscle

AU - Bechshøft, Cecilie J.L.

AU - Jensen, Simon M.

AU - Schjerling, Peter

AU - Andersen, Jesper L.

AU - Svensson, Rene B.

AU - Eriksen, Christian S.

AU - Mkumbuzi, Nonhlanhla S.

AU - Kjaer, Michael

AU - Mackey, Abigail L.

PY - 2019/6

Y1 - 2019/6

N2 - The decline in skeletal muscle regenerative capacity with age is partly attributed to muscle stem cell (satellite cell) dysfunction. Recent evidence has pointed to a strong interaction between myoblasts and fibroblasts, but the influence of age on this interaction is unknown. Additionally, while the native tissue environment is known to determine the properties of myogenic cells in vitro, how the ageing process alters this cell memory has not been established at the molecular level. We recruited 12 young and 12 elderly women, who performed a single bout of heavy resistance exercise with the knee extensor muscles of one leg. 5 days later, muscle biopsies were collected from both legs and myogenic cells and non-myogenic cells were isolated for in vitro experiments with mixed or separated cells, and analysed by immunostaining and RT-PCR. A lower myogenic fusion index was detected in the cells from the old vs. young women, in association with differences in gene expression levels of key myogenic regulatory factors and senescence, which were further altered by performing exercise prior to tissue sampling. Co-culture with non-myogenic cells from the elderly lead to a higher myogenic differentiation index compared to non-myogenic cells from the young. These findings show that the in vitro phenotype and molecular profile of human skeletal muscle myoblasts and fibroblasts is determined by the age and exercise state of the original in vivo environment and help explain how exercise can enhance muscle stem cell function in old age.

AB - The decline in skeletal muscle regenerative capacity with age is partly attributed to muscle stem cell (satellite cell) dysfunction. Recent evidence has pointed to a strong interaction between myoblasts and fibroblasts, but the influence of age on this interaction is unknown. Additionally, while the native tissue environment is known to determine the properties of myogenic cells in vitro, how the ageing process alters this cell memory has not been established at the molecular level. We recruited 12 young and 12 elderly women, who performed a single bout of heavy resistance exercise with the knee extensor muscles of one leg. 5 days later, muscle biopsies were collected from both legs and myogenic cells and non-myogenic cells were isolated for in vitro experiments with mixed or separated cells, and analysed by immunostaining and RT-PCR. A lower myogenic fusion index was detected in the cells from the old vs. young women, in association with differences in gene expression levels of key myogenic regulatory factors and senescence, which were further altered by performing exercise prior to tissue sampling. Co-culture with non-myogenic cells from the elderly lead to a higher myogenic differentiation index compared to non-myogenic cells from the young. These findings show that the in vitro phenotype and molecular profile of human skeletal muscle myoblasts and fibroblasts is determined by the age and exercise state of the original in vivo environment and help explain how exercise can enhance muscle stem cell function in old age.

U2 - 10.1152/ajpcell.00049.2019

DO - 10.1152/ajpcell.00049.2019

M3 - Journal article

C2 - 30917034

VL - 316

SP - C898-C912

JO - American Journal of Physiology: Cell Physiology

JF - American Journal of Physiology: Cell Physiology

SN - 0363-6143

IS - 6

ER -

ID: 215476074