Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice

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Standard

Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice. / Lubberding, Anniek F.; Pereira, Laetitia; Xue, Jianbin; Gottlieb, Lisa A.; Matchkov, Vladimir V.; Gomez, Ana M.; Thomsen, Morten B.

I: Acta Physiologica, Bind 229, Nr. 1, e13444, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Lubberding, AF, Pereira, L, Xue, J, Gottlieb, LA, Matchkov, VV, Gomez, AM & Thomsen, MB 2020, 'Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice', Acta Physiologica, bind 229, nr. 1, e13444. https://doi.org/10.1111/apha.13444

APA

Lubberding, A. F., Pereira, L., Xue, J., Gottlieb, L. A., Matchkov, V. V., Gomez, A. M., & Thomsen, M. B. (2020). Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice. Acta Physiologica, 229(1), [e13444]. https://doi.org/10.1111/apha.13444

Vancouver

Lubberding AF, Pereira L, Xue J, Gottlieb LA, Matchkov VV, Gomez AM o.a. Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice. Acta Physiologica. 2020;229(1). e13444. https://doi.org/10.1111/apha.13444

Author

Lubberding, Anniek F. ; Pereira, Laetitia ; Xue, Jianbin ; Gottlieb, Lisa A. ; Matchkov, Vladimir V. ; Gomez, Ana M. ; Thomsen, Morten B. / Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice. I: Acta Physiologica. 2020 ; Bind 229, Nr. 1.

Bibtex

@article{e2afb8d57cb34424a2d60583f7eff0a2,
title = "Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice",
abstract = "Aim Cardiovascular complications, including cardiac arrhythmias, result in high morbidity and mortality in patients with type-2 diabetes mellitus (T2DM). Clinical and experimental data suggest electrophysiological impairment of the natural pacemaker of the diabetic heart. The present study examined sinoatrial node (SAN) arrhythmias in a mouse model of T2DM and physiologically probed their underlying cause. Methods Electrocardiograms were obtained from conscious diabetic db/db and lean control db/+ mice. In vivo SAN function was probed through pharmacological autonomic modulation with isoprenaline, atropine and carbachol. Blood pressure stability and heart rate variability (HRV) were evaluated. Intrinsic SAN function was evaluated through ex vivo imaging of spontaneous Ca2+ transients in isolated SAN preparations. Results While lean control mice showed constant RR intervals during isoprenaline challenge, the diabetic mice experienced SAN arrhythmias with large RR fluctuations in a dose-dependent manner. These arrhythmias were completely abolished by atropine pre-treatment, while carbachol pretreatment significantly increased SAN arrhythmia frequency in the diabetic mice. Blood pressure and HRV were comparable in db/db and db/+ mice, suggesting that neither augmented baroreceptor feedback nor autonomic neuropathy is a likely arrhythmia mechanism. Cycle length response to isoprenaline was comparable in isolated SAN preparations from db/db and db/+ mice; however, Ca2+ spark frequency was significantly increased in db/db mice compared to db/+ at baseline and after isoprenaline. Conclusion Our results demonstrate a dysfunction of cardiac pacemaking in an animal model of T2DM upon challenge with a beta-adrenergic agonist. Ex vivo, higher Ca2+ spark frequency is present in diabetic mice, which may be directly linked to in vivo arrhythmias.",
keywords = "autonomic nervous system, baroreflex, calcium, electrophysiology, isoprenaline, type-2 diabetes",
author = "Lubberding, {Anniek F.} and Laetitia Pereira and Jianbin Xue and Gottlieb, {Lisa A.} and Matchkov, {Vladimir V.} and Gomez, {Ana M.} and Thomsen, {Morten B.}",
year = "2020",
doi = "10.1111/apha.13444",
language = "English",
volume = "229",
journal = "Acta Physiologica",
issn = "1748-1708",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Aberrant sinus node firing during beta-adrenergic stimulation leads to cardiac arrhythmias in diabetic mice

AU - Lubberding, Anniek F.

AU - Pereira, Laetitia

AU - Xue, Jianbin

AU - Gottlieb, Lisa A.

AU - Matchkov, Vladimir V.

AU - Gomez, Ana M.

AU - Thomsen, Morten B.

PY - 2020

Y1 - 2020

N2 - Aim Cardiovascular complications, including cardiac arrhythmias, result in high morbidity and mortality in patients with type-2 diabetes mellitus (T2DM). Clinical and experimental data suggest electrophysiological impairment of the natural pacemaker of the diabetic heart. The present study examined sinoatrial node (SAN) arrhythmias in a mouse model of T2DM and physiologically probed their underlying cause. Methods Electrocardiograms were obtained from conscious diabetic db/db and lean control db/+ mice. In vivo SAN function was probed through pharmacological autonomic modulation with isoprenaline, atropine and carbachol. Blood pressure stability and heart rate variability (HRV) were evaluated. Intrinsic SAN function was evaluated through ex vivo imaging of spontaneous Ca2+ transients in isolated SAN preparations. Results While lean control mice showed constant RR intervals during isoprenaline challenge, the diabetic mice experienced SAN arrhythmias with large RR fluctuations in a dose-dependent manner. These arrhythmias were completely abolished by atropine pre-treatment, while carbachol pretreatment significantly increased SAN arrhythmia frequency in the diabetic mice. Blood pressure and HRV were comparable in db/db and db/+ mice, suggesting that neither augmented baroreceptor feedback nor autonomic neuropathy is a likely arrhythmia mechanism. Cycle length response to isoprenaline was comparable in isolated SAN preparations from db/db and db/+ mice; however, Ca2+ spark frequency was significantly increased in db/db mice compared to db/+ at baseline and after isoprenaline. Conclusion Our results demonstrate a dysfunction of cardiac pacemaking in an animal model of T2DM upon challenge with a beta-adrenergic agonist. Ex vivo, higher Ca2+ spark frequency is present in diabetic mice, which may be directly linked to in vivo arrhythmias.

AB - Aim Cardiovascular complications, including cardiac arrhythmias, result in high morbidity and mortality in patients with type-2 diabetes mellitus (T2DM). Clinical and experimental data suggest electrophysiological impairment of the natural pacemaker of the diabetic heart. The present study examined sinoatrial node (SAN) arrhythmias in a mouse model of T2DM and physiologically probed their underlying cause. Methods Electrocardiograms were obtained from conscious diabetic db/db and lean control db/+ mice. In vivo SAN function was probed through pharmacological autonomic modulation with isoprenaline, atropine and carbachol. Blood pressure stability and heart rate variability (HRV) were evaluated. Intrinsic SAN function was evaluated through ex vivo imaging of spontaneous Ca2+ transients in isolated SAN preparations. Results While lean control mice showed constant RR intervals during isoprenaline challenge, the diabetic mice experienced SAN arrhythmias with large RR fluctuations in a dose-dependent manner. These arrhythmias were completely abolished by atropine pre-treatment, while carbachol pretreatment significantly increased SAN arrhythmia frequency in the diabetic mice. Blood pressure and HRV were comparable in db/db and db/+ mice, suggesting that neither augmented baroreceptor feedback nor autonomic neuropathy is a likely arrhythmia mechanism. Cycle length response to isoprenaline was comparable in isolated SAN preparations from db/db and db/+ mice; however, Ca2+ spark frequency was significantly increased in db/db mice compared to db/+ at baseline and after isoprenaline. Conclusion Our results demonstrate a dysfunction of cardiac pacemaking in an animal model of T2DM upon challenge with a beta-adrenergic agonist. Ex vivo, higher Ca2+ spark frequency is present in diabetic mice, which may be directly linked to in vivo arrhythmias.

KW - autonomic nervous system

KW - baroreflex

KW - calcium

KW - electrophysiology

KW - isoprenaline

KW - type-2 diabetes

U2 - 10.1111/apha.13444

DO - 10.1111/apha.13444

M3 - Journal article

C2 - 31953990

VL - 229

JO - Acta Physiologica

JF - Acta Physiologica

SN - 1748-1708

IS - 1

M1 - e13444

ER -

ID: 236994645