A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite

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A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite. / Wewer Albrechtsen, Nicolai J; Asmar, Ali; Jensen, Frederik; Törang, Signe; Simonsen, Lene; Kuhre, Rune E; Asmar, Meena; Veedfald, Simon; Plamboeck, Astrid; Knop, Filip K; Vilsbøll, Tina; Madsbad, Sten; Nauck, Michael A; Deacon, Carolyn F; Bülow, Jens; Holst, Jens J; Hartmann, Bolette.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 313, Nr. 3, 01.09.2017, s. E284-E291.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Wewer Albrechtsen, NJ, Asmar, A, Jensen, F, Törang, S, Simonsen, L, Kuhre, RE, Asmar, M, Veedfald, S, Plamboeck, A, Knop, FK, Vilsbøll, T, Madsbad, S, Nauck, MA, Deacon, CF, Bülow, J, Holst, JJ & Hartmann, B 2017, 'A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite', American Journal of Physiology: Endocrinology and Metabolism, bind 313, nr. 3, s. E284-E291. https://doi.org/10.1152/ajpendo.00005.2017

APA

Wewer Albrechtsen, N. J., Asmar, A., Jensen, F., Törang, S., Simonsen, L., Kuhre, R. E., ... Hartmann, B. (2017). A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite. American Journal of Physiology: Endocrinology and Metabolism, 313(3), E284-E291. https://doi.org/10.1152/ajpendo.00005.2017

Vancouver

Wewer Albrechtsen NJ, Asmar A, Jensen F, Törang S, Simonsen L, Kuhre RE o.a. A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite. American Journal of Physiology: Endocrinology and Metabolism. 2017 sep 1;313(3):E284-E291. https://doi.org/10.1152/ajpendo.00005.2017

Author

Wewer Albrechtsen, Nicolai J ; Asmar, Ali ; Jensen, Frederik ; Törang, Signe ; Simonsen, Lene ; Kuhre, Rune E ; Asmar, Meena ; Veedfald, Simon ; Plamboeck, Astrid ; Knop, Filip K ; Vilsbøll, Tina ; Madsbad, Sten ; Nauck, Michael A ; Deacon, Carolyn F ; Bülow, Jens ; Holst, Jens J ; Hartmann, Bolette. / A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite. I: American Journal of Physiology: Endocrinology and Metabolism. 2017 ; Bind 313, Nr. 3. s. E284-E291.

Bibtex

@article{3c9365ac18274f4da5cb26c8c22273d3,
title = "A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite",
abstract = "Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from the gastrointestinal tract. It is best known for its glucose-dependent insulinotropic effects. GLP-1 is secreted in its intact (active) form (7-36NH2) but is rapidly degraded by the dipeptidyl peptidase 4 (DPP-4) enzyme, converting >90{\%} to the primary metabolite (9-36NH2) before reaching the targets via the circulation. Although originally thought to be inactive or antagonistic, GLP-1 9-36NH2 may have independent actions, and it is therefore relevant to be able to measure it. Because reliable assays were not available, we developed a sandwich ELISA recognizing both GLP-1 9-36NH2 and nonamidated GLP-1 9-37. The ELISA was validated using analytical assay validation guidelines and by comparing it to a subtraction-based method, hitherto employed for estimation of GLP-1 9-36NH2 Its accuracy was evaluated from measurements of plasma obtained during intravenous infusions (1.5 pmol × kg(-1) × min(-1)) of GLP-1 7-36NH2 in healthy subjects and patients with type 2 diabetes. Plasma levels of the endogenous GLP-1 metabolite increased during a meal challenge in patients with type 2 diabetes, and treatment with a DPP-4 inhibitor fully blocked its formation. Accurate measurements of the GLP-1 metabolite may contribute to understanding its physiology and role of GLP-1 in diabetes.",
keywords = "Journal Article",
author = "{Wewer Albrechtsen}, {Nicolai J} and Ali Asmar and Frederik Jensen and Signe T{\"o}rang and Lene Simonsen and Kuhre, {Rune E} and Meena Asmar and Simon Veedfald and Astrid Plamboeck and Knop, {Filip K} and Tina Vilsb{\o}ll and Sten Madsbad and Nauck, {Michael A} and Deacon, {Carolyn F} and Jens B{\"u}low and Holst, {Jens J} and Bolette Hartmann",
note = "Copyright {\circledC} 2017 the American Physiological Society.",
year = "2017",
month = "9",
day = "1",
doi = "10.1152/ajpendo.00005.2017",
language = "English",
volume = "313",
pages = "E284--E291",
journal = "American Journal of Physiology: Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "3",

}

RIS

TY - JOUR

T1 - A sandwich ELISA for measurement of the primary glucagon-like peptide-1 metabolite

AU - Wewer Albrechtsen, Nicolai J

AU - Asmar, Ali

AU - Jensen, Frederik

AU - Törang, Signe

AU - Simonsen, Lene

AU - Kuhre, Rune E

AU - Asmar, Meena

AU - Veedfald, Simon

AU - Plamboeck, Astrid

AU - Knop, Filip K

AU - Vilsbøll, Tina

AU - Madsbad, Sten

AU - Nauck, Michael A

AU - Deacon, Carolyn F

AU - Bülow, Jens

AU - Holst, Jens J

AU - Hartmann, Bolette

N1 - Copyright © 2017 the American Physiological Society.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from the gastrointestinal tract. It is best known for its glucose-dependent insulinotropic effects. GLP-1 is secreted in its intact (active) form (7-36NH2) but is rapidly degraded by the dipeptidyl peptidase 4 (DPP-4) enzyme, converting >90% to the primary metabolite (9-36NH2) before reaching the targets via the circulation. Although originally thought to be inactive or antagonistic, GLP-1 9-36NH2 may have independent actions, and it is therefore relevant to be able to measure it. Because reliable assays were not available, we developed a sandwich ELISA recognizing both GLP-1 9-36NH2 and nonamidated GLP-1 9-37. The ELISA was validated using analytical assay validation guidelines and by comparing it to a subtraction-based method, hitherto employed for estimation of GLP-1 9-36NH2 Its accuracy was evaluated from measurements of plasma obtained during intravenous infusions (1.5 pmol × kg(-1) × min(-1)) of GLP-1 7-36NH2 in healthy subjects and patients with type 2 diabetes. Plasma levels of the endogenous GLP-1 metabolite increased during a meal challenge in patients with type 2 diabetes, and treatment with a DPP-4 inhibitor fully blocked its formation. Accurate measurements of the GLP-1 metabolite may contribute to understanding its physiology and role of GLP-1 in diabetes.

AB - Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from the gastrointestinal tract. It is best known for its glucose-dependent insulinotropic effects. GLP-1 is secreted in its intact (active) form (7-36NH2) but is rapidly degraded by the dipeptidyl peptidase 4 (DPP-4) enzyme, converting >90% to the primary metabolite (9-36NH2) before reaching the targets via the circulation. Although originally thought to be inactive or antagonistic, GLP-1 9-36NH2 may have independent actions, and it is therefore relevant to be able to measure it. Because reliable assays were not available, we developed a sandwich ELISA recognizing both GLP-1 9-36NH2 and nonamidated GLP-1 9-37. The ELISA was validated using analytical assay validation guidelines and by comparing it to a subtraction-based method, hitherto employed for estimation of GLP-1 9-36NH2 Its accuracy was evaluated from measurements of plasma obtained during intravenous infusions (1.5 pmol × kg(-1) × min(-1)) of GLP-1 7-36NH2 in healthy subjects and patients with type 2 diabetes. Plasma levels of the endogenous GLP-1 metabolite increased during a meal challenge in patients with type 2 diabetes, and treatment with a DPP-4 inhibitor fully blocked its formation. Accurate measurements of the GLP-1 metabolite may contribute to understanding its physiology and role of GLP-1 in diabetes.

KW - Journal Article

U2 - 10.1152/ajpendo.00005.2017

DO - 10.1152/ajpendo.00005.2017

M3 - Journal article

C2 - 28420649

VL - 313

SP - E284-E291

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

IS - 3

ER -

ID: 182973352