A porcine model of haematogenous brain infectionwith staphylococcus aureus

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskning

Standard

A porcine model of haematogenous brain infectionwith staphylococcus aureus. / Astrup, Lærke Boye; Agerholm, Jørgen Steen; Nielsen, Ole Lerberg; Jensen, Henrik Michael Elvang; Leifsson, Páll S.; Iburg, Tine M.

2012.

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskning

Harvard

Astrup, LB, Agerholm, JS, Nielsen, OL, Jensen, HME, Leifsson, PS & Iburg, TM 2012, 'A porcine model of haematogenous brain infectionwith staphylococcus aureus'.

APA

Astrup, L. B., Agerholm, J. S., Nielsen, O. L., Jensen, H. M. E., Leifsson, P. S., & Iburg, T. M. (2012). A porcine model of haematogenous brain infectionwith staphylococcus aureus.

Vancouver

Astrup LB, Agerholm JS, Nielsen OL, Jensen HME, Leifsson PS, Iburg TM. A porcine model of haematogenous brain infectionwith staphylococcus aureus. 2012.

Author

Astrup, Lærke Boye ; Agerholm, Jørgen Steen ; Nielsen, Ole Lerberg ; Jensen, Henrik Michael Elvang ; Leifsson, Páll S. ; Iburg, Tine M. / A porcine model of haematogenous brain infectionwith staphylococcus aureus. 1 s.

Bibtex

@conference{6f52f560ed5b44cb98cded9771c32c6c,
title = "A porcine model of haematogenous brain infectionwith staphylococcus aureus",
abstract = "A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS Astrup L{\ae}rke1, Agerholm J{\o}rgen1, Nielsen Ole1, Jensen Henrik1, Leifsson P{\'a}ll1, Iburg Tine2.1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmarkboye@life.ku.dk2: National Veterinary Institute, Uppsala, SwedenIntroductionStaphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection.Materials and MethodsFour pigs had two intravenous catheters inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue thromboemboli (two pigs). The venous catheter was used for blood sampling before, during and after inoculation. The pigs were euthanized either 24 or 48 hours after inoculation. The brains were collected and examined histologically. ResultsWe describe unifocal suppurative encephalitis 48 hours after inoculation with autologoue S. aureus thromoemboli. The other pigs developed mild diffuse cerebral oedema. The blood analyses showed a short period of bacteraemia.Conclusion Our pilot study proves it possible to establish embolic infection in the porcine brain through the normal circulatory pathway, when autologue thromboemboli are used. This was hitherto thought impossible due to the presence of rete mirabilis in this species.",
keywords = "Former LIFE faculty, Animal models, brain, Staphylococcus aureus",
author = "Astrup, {L{\ae}rke Boye} and Agerholm, {J{\o}rgen Steen} and Nielsen, {Ole Lerberg} and Jensen, {Henrik Michael Elvang} and Leifsson, {P{\'a}ll S.} and Iburg, {Tine M.}",
year = "2012",
language = "English",

}

RIS

TY - ABST

T1 - A porcine model of haematogenous brain infectionwith staphylococcus aureus

AU - Astrup, Lærke Boye

AU - Agerholm, Jørgen Steen

AU - Nielsen, Ole Lerberg

AU - Jensen, Henrik Michael Elvang

AU - Leifsson, Páll S.

AU - Iburg, Tine M.

PY - 2012

Y1 - 2012

N2 - A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1, Leifsson Páll1, Iburg Tine2.1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmarkboye@life.ku.dk2: National Veterinary Institute, Uppsala, SwedenIntroductionStaphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection.Materials and MethodsFour pigs had two intravenous catheters inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue thromboemboli (two pigs). The venous catheter was used for blood sampling before, during and after inoculation. The pigs were euthanized either 24 or 48 hours after inoculation. The brains were collected and examined histologically. ResultsWe describe unifocal suppurative encephalitis 48 hours after inoculation with autologoue S. aureus thromoemboli. The other pigs developed mild diffuse cerebral oedema. The blood analyses showed a short period of bacteraemia.Conclusion Our pilot study proves it possible to establish embolic infection in the porcine brain through the normal circulatory pathway, when autologue thromboemboli are used. This was hitherto thought impossible due to the presence of rete mirabilis in this species.

AB - A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1, Leifsson Páll1, Iburg Tine2.1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmarkboye@life.ku.dk2: National Veterinary Institute, Uppsala, SwedenIntroductionStaphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection.Materials and MethodsFour pigs had two intravenous catheters inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue thromboemboli (two pigs). The venous catheter was used for blood sampling before, during and after inoculation. The pigs were euthanized either 24 or 48 hours after inoculation. The brains were collected and examined histologically. ResultsWe describe unifocal suppurative encephalitis 48 hours after inoculation with autologoue S. aureus thromoemboli. The other pigs developed mild diffuse cerebral oedema. The blood analyses showed a short period of bacteraemia.Conclusion Our pilot study proves it possible to establish embolic infection in the porcine brain through the normal circulatory pathway, when autologue thromboemboli are used. This was hitherto thought impossible due to the presence of rete mirabilis in this species.

KW - Former LIFE faculty

KW - Animal models

KW - brain

KW - Staphylococcus aureus

M3 - Conference abstract for conference

ER -

ID: 41838188