Membrane Trafficking – University of Copenhagen

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English > Research > Ion Channel Group > Membrane Trafficking

When examined at the subcellular level, ion channels display an impressive precision in their localization. Yet, highly related ion channels localize to different subcellular compartments. And still, ion channel localization is dynamic and can change dramatically in response to different physiological conditions.

The precision, the diversity and the dynamics of ion channel localization are all critical parameters for proper regulation of essential processes such as neuronal signaling and transepithelial transport.

In the Membrane Trafficking Lab, we study the molecular and cellular machinery responsible for the mosaic distribution of ion channels in polarized cells and how the molecular and cellular machinery is regulated. The goal is to understand how highly complex cells such as epithelial and neuronal cells solve the demanding task of having the right number of the right ion channels at the right spot at the right time.

We study these questions using epithelial cell lines and primary cultured hippocampal neurons as model systems. Technically, we are fond of various imaging methods with a current focus on dynamic, live-cell imaging studies, where we directly follow polarized transport and plasma membrane dynamics of fluorescently tagged ion channels in living epithelial and neuronal cells. In addition, we use a variety of molecular biological, biochemical and electrophysiological techniques.

Group members

Hanne Borger Rasmussen, MSc, PhD
Associate Professor
Phone: +45 3532 7557
See CV and publication list
Louise Leth Hefting, MSc
PhD Student
Phone: +45 3533 0967
See CV and publication list
Martin Larsen, BSc
Master Student

Christoffer Badsted, BSc

Master Student

Selected publications:

Benned-Jensen, T; Kordt Christensen, R, Denti, F, Perrier, JF, Rasmussen, HB, Olesen SP. Live imaging of Kv7.2/7.3 cell surface dynamics at the axon initial segment: high steady state stability and calpain-dependent excitotoxic downregulation revealed. J. Neurosci., in press.

Andersen, M. N. et al. Protein kinase A stimulates Kv7.1 surface expression by regulating Nedd4-2-dependent endocytic trafficking. Am. J. Physiol., Cell Physiol. 309, C693–706 (2015)

Andersen, M. N. et al. A phosphoinositide 3-kinase (PI3K)-serum- and glucocorticoid-inducible kinase 1 (SGK1) pathway promotes Kv7.1 channel surface expression by inhibiting Nedd4-2 protein. J. Biol. Chem. 288, 36841–36854 (2013).

Andersen, M. N., Olesen, S.-P. & Rasmussen, H. B. Kv7.1 surface expression is regulated by epithelial cell polarization. Am. J. Physiol., Cell Physiol. 300, C814–824 (2011).

Jensen, C. S., Rasmussen, H. B. & Misonou, H. Neuronal trafficking of voltage-gated potassium channels. Mol. Cell. Neurosci. 48, 288–297 (2011).