Research techniques – University of Copenhagen

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English > Research > Cardiac Physiology > Techniques

Research techniques

The following are some of the techniques implemented in the laboratory

  Two-electrode voltage-clamp. Electrophysiological measurements of proteins expressed in Xenopus oocytes.
Langendorff Langendorff experimentation. Electrical and hemodynamic monitoring of explanted beating hearts in a perfusion system.
Arial and ventricular action potential Action potential recordings of beating intact cardiac tissue – human, horse, rat, guinea pig and mouse.
Electrophysiology Patch-clamping of native cardiomyocytes and of culture cells heterologously expressing proteins of interest.
Biochemistry

Biochemistry. Western blotting, co-immunoprecipitation, and GST pull-down.
Molecular biology. Plasmid clonings, shRNA evaluation.

Real-time PCR

Real-time PCR (qPCR).

Electrodiogram

Electrocardiogram (ECG)