Research techniques – University of Copenhagen

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Research techniques

Act4Life is skilled in performing human intervention studies involving different types of physical exercise.

We do detailed, human, metabolic phenotyping and e.g. determine cardiorespiratory fitness by graded exercise test and indirect calorimetry, body composition by DXA-scanning, and peripheral insulin sensitivity by hyperinsulinemic, euglycemic clamp.


We sample blood, urine, saliva, faeces, adipose tissue and skeletal muscle and also master the microdialysis technique in human adipose tissue and muscle. Physical activity in free-living humans is estimated via heart rate monitors, accelerometers and questionnaires. We also perform appetite and oral glucose tolerance tests and measure sleep via accelerometers and questionnaires.

We quantitate glucose uptake in various human adipose tissue and muscle depots via PET/CT-scanning and abdominal fat and femoral muscle mass via MRI-scanning in collaboration with the Department of Clinical Physiology, Nuclear Medicine and PET, Rigshospitalet, Denmark. Activity level in different domains of everyday life is evaluated via smartphones in collaboration with the Department of Applied Mathematics and Computer Science, Technical University of Denmark, Denmark.